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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 110, No. 8, 2015, pp. 1024-1034
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Bioline Code: oc15139
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 110, No. 8, 2015, pp. 1024-1034
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Antileishmanial activity of the essential oil from Tetradenia riparia obtained in different seasons
Cardoso, Bruna Muller; Mello, Tatiane França Perles de; Lopes, Sara Negrão; Demarchi, Izabel Galhardo; Lera, Daniele Stefani Lopes; Pedroso, Raíssa Bocchi; Cortez, Diogenes Aparício; Gazim, Zilda Cristiani; Aristides, Sandra Mara Alessi; Silveira, Thais Gomes Verzignassi & Lonardoni, Maria Valdrinez Campana
Abstract
The herbaceous shrub Tetradenia riparia has been traditionally used to treat inflammatory and infectious diseases.
Recently, a study showed that T. riparia essential oil (TrEO) obtained in summer has antileishmanial effects,
although these results could be influenced by seasonal variation. This study evaluated the activity of the TrEO
obtained in different seasons against Leishmania (Leishmania) amazonensis , in vitro and in vivo. The compounds
in the TrEO were analysed by gas chromatography-mass spectrometry; terpenoids were present and oxygenated
sesquiterpenes were the majority compounds (55.28%). The cytotoxicity and nitric oxide (NO) production were
also tested after TrEO treatment. The TrEO from all seasons showed a 50% growth inhibitory concentration for
promastigotes of about 15 ng/mL; at 30 ng/mL and 3 ng/mL, the TrEO reduced intracellular amastigote infection,
independently of season. The TrEO from plants harvested in summer had the highest 50% cytotoxic concentration,
1,476 ng/mL for J774.A1 macrophages, and in spring (90.94 ng/mL) for murine macrophages. NO production did not
change in samples of the TrEO from different seasons. The antileishmanial effect in vivo consisted of a reduction
of the parasite load in the spleen. These results suggest that the TrEO has potential effects on L. (L.) amazonensis,
consonant with its traditional use to treat parasitic diseases.
Keywords
Tetradenia riparia; essential oil; Leishmania (Leishmania) amazonensis; antileishmanial activity; nitric oxide
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