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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 111, No. 1, 2016, pp. 20-29
Bioline Code: oc16002
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 1, 2016, pp. 20-29

 en Mayaro virus and dengue virus 1 and 4 natural infection in culicids from Cuiabá, state of Mato Grosso, Brazil
Serra, Otacília Pereira; Cardoso, Belgath Fernandes; Ribeiro, Ana Lúcia Maria; Santos, Fábio Alexandre Leal dos & Slhessarenko, Renata Dezengrini

Abstract

This study aimed to verify the diversity of Culicidae species and their frequency of infection with flaviviruses and alphaviruses in Cuiabá, state of Mato Grosso, Brazil. Mosquitoes were captured with Nasci aspirators and hand net in 200 census tracts, identified alive at species level and pooled in one-20 (11,090 mosquitoes, 14 species). Female pools (n = 610) were subjected to multiplex seminested-reverse transcription-polymerase chain reaction (RT-PCR) for 11 flavivirus and five alphavirus. Positive pools were tested by single RT-PCR followed by nucleotide sequencing, by RT-PCR for E1 gene [Mayaro virus (MAYV)] and by inoculation in Vero cells (MAYV) or C6/36 cells (flaviviruses). One/171 Aedes aegypti check for this species in other resources was positive for dengue virus (DENV)-1, 12/403 Culex quinquefasciatus check for this species in other resources , and four/171 Ae. aegypti for MAYV, which was isolated from two pools containing two nonengorged females of Ae. aegypti and two of Cx. quinquefasciatus. DENV-4 was detected in 58/171 pools of Ae. aegytpi, 105/403 Cx. quinquefasciatus, two/five Psorophora check for this species in other resources sp., two/11 Psorophora varipes check for this species in other resources / Psorophora albigenu check for this species in other resources , one/one Sabethes chloropterus check for this species in other resources , two/five Culex bidens check for this species in other resources / Culex interfor check for this species in other resources , and one/one Aedes check for this species in other resources sp. DENV-4 was isolated from two pools containing three and 16 nonengorged Cx. quinquefasciatus females. Phylogenetic analysis revealed MAYV belongs to genotype L, clustering with human samples of the virus previously identified in the city. Cuiabá has biodiversity and ecosystem favourable for vector proliferation, representing a risk for arbovirus outbreaks.

Keywords
dengue virus; Mayaro virus; entomological surveillance

 
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