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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 111, No. 1, 2016, pp. 37-42
Bioline Code: oc16004
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 1, 2016, pp. 37-42

 en Polyphasic characterisation of Burkholderia cepacia check for this species in other resources complex species isolated from children with cystic fibrosis
Vicenzi, Fernando José; Pillonetto, Marcelo; Souza, Helena Aguilar Peres Homem de Mello de; Palmeiro, Jussara Kasuko; Riedi, Carlos Antônio; Rosario-Filho, Nelson Augusto & Nelson Augusto, Libera Maria

Abstract

Cystic fibrosis (CF) patients with Burkholderia cepacia check for this species in other resources complex (Bcc) pulmonary infections have high morbidity and mortality. The aim of this study was to compare different methods for identification of Bcc species isolated from paediatric CF patients. Oropharyngeal swabs from children with CF were used to obtain isolates of Bcc samples to evaluate six different tests for strain identification. Conventional (CPT) and automatised (APT) phenotypic tests, polymerase chain reaction (PCR)-recA, restriction fragment length polymorphism-recA, recA sequencing, and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) were applied. Bacterial isolates were also tested for antimicrobial susceptibility. PCR-recA analysis showed that 36 out of the 54 isolates were Bcc. Kappa index data indicated almost perfect agreement between CPT and APT, CPT and PCR-recA, and APT and PCR-recA to identify Bcc, and MALDI-TOF and recA sequencing to identify Bcc species. The recA sequencing data and the MALDI-TOF data agreed in 97.2% of the isolates. Based on recA sequencing, the most common species identified were Burkholderia cenocepacia check for this species in other resources IIIA (33.4%), Burkholderia vietnamiensis check for this species in other resources (30.6%), B. cenocepacia IIIB (27.8%), Burkholderia multivorans check for this species in other resources (5.5%), and B. cepacia (2.7%). MALDI-TOF proved to be a useful tool for identification of Bcc species obtained from CF patients, although it was not able to identify B. cenocepacia subtypes.

Keywords
Burkholderia cepacia complex; cystic fibrosis; lung disease; Gram-negative bacterial infection; DNA sequence analyses; matrix-assisted laser desorption-ionisation mass spectrometry

 
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