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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 111, No. 1, 2016, pp. 51-58
Bioline Code: oc16006
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 1, 2016, pp. 51-58

 en First description of Candida nivariensis check for this species in other resources in Brazil: antifungal susceptibility profile and potential virulence attributes
Figueiredo-Carvalho, Maria Helena Galdino; Ramos, Livia de Souza; Barbedo, Leonardo Silva; Chaves, Alessandra Leal da Silva; Muramoto, Ilda Akemi; Santos, André Luis Souza dos; Almeida-Paes, Rodrigo & Zancopé-Oliveira, Rosely Maria

Abstract

This study evaluated the antifungal susceptibility profile and the production of potential virulence attributes in a clinical strain of Candida nivariensis check for this species in other resources for the first time in Brazil, as identified by sequencing the internal transcribed spacer (ITS)1-5.8S-ITS2 region and D1/D2 domains of the 28S of the rDNA. For comparative purposes, tests were also performed with reference strains. All strains presented low planktonic minimal inhibitory concentrations (PMICs) to amphotericin B (AMB), caspofungin (CAS), and voriconazole. However, our strain showed elevated planktonic MICs to posaconazole (POS) and itraconazole, in addition to fluconazole resistance. Adherence to inert surfaces was conducted onto glass and polystyrene. The biofilm formation and antifungal susceptibility on biofilmgrowing cells were evaluated by crystal violet staining and a XTT reduction assay. All fungal strains were able to bind both tested surfaces and form biofilm, with a binding preference to polystyrene (p < 0.001). AMB promoted significant reductions (≈50%) in biofilm production by our C. nivariensis strain using both methodologies. This reduction was also observed for CAS and POS, but only in the XTT assay. All strains were excellent protease producers and moderate phytase producers, but lipases were not detected. This study reinforces the pathogenic potential of C. nivariensis and its possible resistance profile to the azolic drugs generally used for candidiasis management.

Keywords
Candida nivariensis; Brazil; antifungal resistance; hydrolytic enzymes; adherence; biofilm

 
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