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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 111, No. 2, 2016, pp. 114-119
Bioline Code: oc16015
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 2, 2016, pp. 114-119

 en Frequency and molecular characterisation of Entamoeba histolytica check for this species in other resources , Entamoeba dispar check for this species in other resources , Entamoeba moshkovskii check for this species in other resources , and Entamoeba hartmanni check for this species in other resources in the context of water scarcity in northeastern Brazil
Calegar, Deiviane Aparecida; Nunes, Beatriz Coronato; Monteiro, Kerla Joeline Lima; Santos, Jéssica Pereira dos; Toma, Helena Keiko; Gomes, Tais Ferreira; Lima, Marli Maria; Bóia, Márcio Neves & Carvalho-Costa, Filipe Anibal

Abstract

This study aimed to estimate the frequency, associated factors, and molecular characterisation of Entamoeba histolytica check for this species in other resources , Entamoeba dispar, Entamoeba moshkovskii check for this species in other resources , and Entamoeba hartmanni check for this species in other resources infections. We performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. Faecal samples were processed through flotation and centrifugation methods. E. histolytica, E. dispar, E. moshkovskii, and E. hartmanni were identified by nested-polymerase chain reaction (PCR). The overall prevalence of infection was 22/213 (10.3%). The infection rate among subjects who drink rainwater collected from roofs in tanks was higher than the rate in subjects who drink desalinated water pumped from wells; similarly, the infection rate among subjects who practice open defecation was significantly higher than that of subjects with latrines. Out of the 22 samples positive for morphologically indistinguishable Entamoeba species, the differentiation by PCR was successful for 21. The species distribution was as follows: 57.1% to E. dispar, 23.8% to E. histolytica, 14.3% to E. histolytica and E. dispar, and 4.8% E. dispar and E. hartmanni. These data suggest a high prevalence of asymptomatic infection by the group of morphologically indistinguishable Entamoeba histolytica/dispar/moshkovskii complex and E. hartmanni species. In this context of water scarcity, the sanitary and socioenvironmental characteristics of the region appear to favour transmission.

Keywords
parasites; protozoa; entamoebiasis; epidemiology; Brazil

 
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