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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 111, No. 4, 2016, pp. 267-270
Bioline Code: oc16036
Full paper language: English
Document type: Short Communication
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 4, 2016, pp. 267-270

 en The identification and differentiation of the Candida parapsilosis check for this species in other resources complex species by polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer region of the rDNA
Barbedo, Leonardo Silva; Figueiredo-Carvalho, Maria Helena Galdino; Muniz, Mauro de Medeiros & Zancopé-Oliveira, Rosely Maria

Abstract

Currently, it is accepted that there are three species that were formerly grouped under Candida parapsilosis check for this species in other resources : C. parapsilosis sensu stricto, Candida orthopsilosis check for this species in other resources , and Candida metapsilosis check for this species in other resources . In fact, the antifungal susceptibility profiles and distinct virulence attributes demonstrate the differences in these nosocomial pathogens. An accurate, fast, and economical identification of fungal species has been the main goal in mycology. In the present study, we searched sequences that were available in the GenBank database in order to identify the complete sequence for the internal transcribed spacer (ITS)1-5.8S-ITS2 region, which is comprised of the forward and reverse primers ITS1 and ITS4. Subsequently, an in silico polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to differentiate the C. parapsilosis complex species. Ninety-eight clinical isolates from patients with fungaemia were submitted for analysis, where 59 isolates were identified as C. parapsilosis sensu stricto, 37 were identified as C. orthopsilosis, and two were identified as C. metapsilosis. PCR-RFLP quickly and accurately identified C. parapsilosis complex species, making this method an alternative and routine identification system for use in clinical mycology laboratories.

Keywords
Candida parapsilosis complex; PCR-RFLP; fungaemia

 
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