Salivary gland polytene chromosomes of 4th instar
Anopheles darlingi
Root were examined from multiple locations
in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included
changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four
new paracentric inversions, two on the right arm of chromosome 3 and two on the left arm of chromosome 3 that
were found in multiple locations. A total of 18 inversions on the X (n = 1) chromosome, chromosome 2 (n = 7) and 3
(n = 11) were scored for 83 individuals from Manaus, Macapá and Porto Velho municipalities. The frequency of 2Ra
inversion karyotypes in Manaus shows significant deficiency of heterozygotes (p < 0.0009). No significant linkage
disequilibrium was found between inversions on chromosome 2 and 3. We hypothesize that at least two sympatric
subpopulations exist within the
An. darlingi population at Manaus based on inversion frequencies.
