During its life cycle
Leishmania spp. face several stress conditions that can cause DNA damages. Base Excision
Repair plays an important role in DNA maintenance and it is one of the most conserved mechanisms in all living
organisms. DNA repair in trypanosomatids has been reported only for Old World
Leishmania species. Here the AP
endonuclease from
Leishmania (L.) amazonensis was cloned, expressed in
Escherichia coli
mutants defective on the
DNA repair machinery, that were submitted to different stress conditions, showing ability to survive in comparison
to the triple null mutant parental strain BW535. Phylogenetic and multiple sequence analyses also confirmed that
LAMAP belongs to the AP endonuclease class of proteins.