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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 111, No. 6, 2016, pp. 411-413
Bioline Code: oc16061
Full paper language: English
Document type: Note
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 6, 2016, pp. 411-413

 en Low impact to fixed cell processing aiming transmission electron microscopy
Barth, Ortrud Monika; Nunes da Silva, Marcos Alexandre & Barreto-Vieira, Debora Ferreira

Abstract

In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.

Keywords
cell fixation; transmission electron microscopy; low impact; DENV

 
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