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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 7, 2016, pp. 454-459
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Bioline Code: oc16068
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 7, 2016, pp. 454-459
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Evaluation of crystal violet decolorization assay for minimal inhibitory concentration detection of primary antituberculosis drugs against Mycobacterium tuberculosis isolates*
Coban, Ahmet Yilmaz; Akbal, Ahmet Ugur; Uzun, Meltem; Cayci, Yeliz Tanriverdi; Birinci, Asuman & Durupinar, Belma
Abstract
In this study we evaluated the crystal violet decolorization assay (CVDA) for detection of minimum inhibitory concentration
(MIC) of antituberculosis drugs. 53 isolates were tested in this study and 13 of them were multidrug resistant
(MDR) isolates. The antibiotics concentrations were 2-0.06 mg/L for isoniazid (INH) and rifampicin (RIF) and were 16-
0.25 mg/L for streptomycin (STM) and ethambutol (EMB). Crystal violet (CV-25 mg/L) was added into the microwells on
the seventh day of incubation and incubation was continued until decolorization. Decolorization of CV was the predictor
of bacterial growth. Overall agreements for four drugs were detected as 98.1%, and the average time was detected as
9.5 ± 0.89 day after inoculation. One isolate for INH and two isolates for STM were determined resistant in the reference
method, but susceptible by the CVDA. One isolate was susceptible to EMB by the reference method, but resistant by the
CVDA. All results were concordant for RIF. This study shows that CVDA is a rapid, reliable and suitable for determination
of MIC values of Mycobacterium tuberculosis. And it can be used easily especially in countries with limited-sources.
Keywords
Mycobacterium tuberculosis; antituberculosis drugs; susceptibility testing; multi drug resistance; crystal violet decolorization assay
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