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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 8, 2016, pp. 532-534
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Bioline Code: oc16080
Full paper language: English
Document type: Short Communication
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 8, 2016, pp. 532-534
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Ultrastructure of Zika virus particles in cell cultures
Barreto-Vieira, Debora Ferreira; Barth, Ortrud Monika; da Silva, Marcos Alexandre Nunes; Santos, Carolina Cardoso; Santos, Aline da Silva; Filho, Joaquim Batista F. & de Filippis, Ana Maria Bispo
Abstract
Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015.
The introduction of ZIKV brought a strong impact to public health in Brazil. It is of utmost importance to identify a
susceptible cell line that will enable the isolation and identification of the virus from patient samples, viral mass production,
and testing of drug and vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction
diagnosis for detecting the viral genome, virus isolation in cell lines was useful in order to study the structure of the
viral particle and its behaviour inside cells. Analysis of ZIKV infected cell lines was achieved using transmission electron
microscopy (TEM). Blood was obtained from a Brazilian patient during the first days after presenting with signs of
the disease, and ZIKV from the patient’s blood was isolated in the C6/36 mosquito cell line. Afterwards, Vero cells were
inoculated with the viral suspension, fixed six days after inoculation, embedded in polymers, and ultra-thin cut. Like
dengue viruses, this flavivirus showed numerous virus particles present inside cellular vesicles thereby confirming the
susceptibility of the Vero cell line to ZIKV replication. TEM is a unique technique available to make the virus visible.
Keywords
Zika virus; virus particles; cell culture; transmission electron microscopy
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