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Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients
Soto, Yudira; Limia, Celia Maria; González, Licet; Grá, Bienvenido; Hano, Olga Marina; Martínez, Pedro Ariel & Kourí, Vivian
Abstract
The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The
aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban
patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma,
18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by
quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16,
-18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed.
Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of
adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases.
An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio =
4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral
loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular
evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients.
Keywords
human papillomavirus; colorectal cancer; real-time PCR
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