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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 12, 2016, pp. 745-749
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Bioline Code: oc16119
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 12, 2016, pp. 745-749
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Surveillance of antiviral resistance markers in Argentina: detection of E119V neuraminidase mutation in a post-treatment immunocompromised patient
Pontoriero, Andrea; Avaro, Martín; Benedetti, Estefania; Russo, Mara; Czech, Andrea; Periolo, Natalia; Campos, Ana; Zamora, Ana & Baumeister, Elsa
Abstract
Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the
main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase
(NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir administration is E119V mutation.
We describe the emergence of a mixed viral population with the E119E/V mutation in the NA protein sequence in
a post-treatment influenza sample collected from an immunocompromised patient in Argentina. This substitution was
identified by a real-time reverse transcriptase polymerase chain reaction (RT-PCR) protocol and was confirmed by direct
Sanger sequencing of the original sample. In 2014, out of 1140 influenza samples received at the National Influenza
Centre, 888 samples (78%) were A(H3N2) strains, 244 (21.3%) were type B strains, and 8 (0.7%) were A(H1N1)pdm09
strains. Out of 888 A(H3N2) samples, 842 were tested for the E119V substitution by quantitative RT-PCR: 841 A(H3N2)
samples had the wild-type E119 genotype and in one sample, a mixture of viral E119/ V119 subpopulations was detected.
Influenza virus surveillance and antiviral resistance studies can lead to better decisions in health policies and help in
medical treatment planning, especially for severe cases and immunocompromised patients.
Keywords
influenza resistance; Argentina; E119V substitution; surveillance
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