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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 111, No. 12, 2016, pp. 757-764
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Bioline Code: oc16121
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 111, No. 12, 2016, pp. 757-764
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A non-hepatotropic parasite infection increases mortality in the acetaminophen-induced acute liver failure murine model: possible roles for IL-5 and IL-6
León-Nava, Marco A de; Álvarez-Delgado, Carolina; Donis-Maturano, Luis; Hernández-Ruiz, Joselin; Manjarrez-Reyna, Aaron N; Cruz-Avilés, Edgar; Leon-Cabrera, Sonia; Morales-Montor, Jorge; Fragoso, José M & Escobedo, Galileo
Abstract
We evaluated the effects of a non-hepatotropic parasite infection (Taenia crassiceps) on the outcome of acetaminophen-
induced acute liver failure in mice. Uninfected and T. crassiceps infected mice orally received either 300 mg/kg
acetaminophen or water as vehicle (n = 5 per group). Survival analysis, hepatocyte necrosis, alanine aminotransferase
(ALT) levels, CYP2E1 protein, interleukin (IL-) 5, and IL-6 were assessed for all groups. All infected mice died within
16 h after exposure to acetaminophen (Tc+APAP group), whereas only one-third of uninfected animals exposed to
acetaminophen (APAP group) died. Uninfected (Control group) and infected (Tc group) mice that received the vehicle
showed no liver damage. Tc+APAP mice exhibited massive liver necrosis characterised by marked balloning degeneration
of hepatocytes and higher serum ALT compared to Control, Tc, and APAP animals. Liver tissue from Tc+APAP
mice also displayed increased expression of CYP2E1 protein and higher mRNA and protein levels of IL-5 and IL-6
compared to the other groups. These findings suggest that non-hepatotropic parasite infections may increase mortality
following acute liver failure by promoting hepatocyte necrosis via IL-5 and IL-6-dependent CYP2E1 overproduction.
This study identifies new potential risk factors associated with severe acute liver failure in patients.
Keywords
acute liver failure; acetaminophen; parasite infection; Taenia crassiceps; liver disease; interleukin
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