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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 112, No. 1, 2017, pp. 75-78
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Bioline Code: oc17009
Full paper language: English
Document type: Short Communication
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 112, No. 1, 2017, pp. 75-78
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First description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, Brazil
Araujo-Pereira, Thais de; Pita-Pereira, Daniela de; Boité, Mariana Côrtes; Melo, Myllena; Costa-Rego, Taiana Amancio da; Fuzari, Andressa Alencastre; Brazil, Reginaldo Peçanha & Britto, Constança
Abstract
Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon,
a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain
reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subgenus in 12
out of 173 sandflies captured in the municipality of Rio Branco, Acre state, revealing a positivity of 6.94%. By sequencing
the Leishmania 234 bp-hsp70 amplified products from positive samples, infection by L. (V.) braziliensis
was confirmed in five sandflies: one Evandromyia saulensis, three Trichophoromyia auraensis and one Pressatia
sp. The finding of L. (Viannia) DNA in two Ev. saulensis corresponds to the first record of possible infection associated
with this sandfly. Moreover, our study reveals for the first time in Brazil, Th. auraensis and Pressatia sp.
infected by L. (Viannia) parasites.
Keywords
sandfly; Leishmania Viannia; molecular diagnosis; Acre.
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