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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 112, No. 3, 2017, pp. 220-223
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Bioline Code: oc17029
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 112, No. 3, 2017, pp. 220-223
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Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
Lima, Lyana Rodrigues Pinto; Silva, Amanda Perse da; Schmidt-Chanasit, Jonas & Paula, Vanessa Salete de
Abstract
The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity
and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical
specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic
standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed
and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.
Keywords
real time PCR; synthetic curve; herpes
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