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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 112, No. 6, 2017, pp. 404-410
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Bioline Code: oc17053
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 112, No. 6, 2017, pp. 404-410
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Enterocytozoon bieneusi detected by molecular methods in raw sewage and treated effluent from a combined system in Brazil
Yamashiro, Sandra; Fiuza, Vagner Ricardo da Silva; Teixeira, Ângela Therezinha Lauand Sampaio; Branco, Nilson; Levy, Carlos Emílio; Castro, Isabel Cristina Vidal Siqueira de & Franco, Regina Maura Bueno
Abstract
BACKGROUND Enterocytozoon bieneusi are the most common microsporidia associated with different clinical manifestations
such as diarrhoea, respiratory tract inflammation and acalculous cholecystitis, especially in immunocompromised patients.
Infection usually occurs by ingestion of food and water contaminated with spores, but can also result from direct contact with
spores through broken skin, eye lesions, and sexual transmission, depending on the microsporidian species. Although there are
reports of E. bieneusi found in humans and animals in Brazil, there are no published studies of environmental samples examined
by molecular methods.
OBJECTIVES The purpose of this study was to verify the presence of E. bieneusi in raw sewage and treated effluent from a
combined system by molecular methods.
METHODS Raw sewage and treated effluent samples collected from a combined system were analysed for the presence of E.
bieneusi using the internal transcriber spacer (ITS) region of E. bieneusi by nested polymerase chain reaction.
FINDINGS The analysis revealed E. bieneusi presence and a novel genotype (EbRB) in one raw sewage sample and one treated effluent.
MAIN CONCLUSIONS The presence of E. bieneusi in final effluent indicates that the combined system may not remove
microsporidian spores. This study is the first report of E. bieneusi in environmental samples in Brazil.
Keywords
sewage; Enterocytozoon bieneusi; PCR; treated effluent; combined system; Brazil
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