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Human papillomavirus type distribution and HPV16 intratype diversity in southern Brazil in women with and without cervical lesions
Oliveira, Gisele R de; Vieira, Valdimara C; Ávila, Emiliana C; Finger-Jardim, Fabiana; Caldeira, Thaís DM; Gatti, Fabiane AA; Gonçalves, Carla V; Oliveira, Sandro G; Hora, Vanusa P da; Soares, Marcelo A & Martinez, Ana MB de
Abstract
BACKGROUND Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages)
are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer.
OBJECTIVES This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in
southern Brazil in women with normal cytology or intraepithelial lesions.
METHODS HPV typing was determined by L1 gene sequencing. To identify HPV16 variants, the LCR and E6 regions were
sequenced, and characteristic single nucleotide variants were identified.
FINDINGS A total of 445 samples were studied, with 355 from cervical scrapes and 90 from cervical biopsies. HPV was detected
in 24% and 91% of these samples, respectively. The most prevalent HPV types observed were 16 (cervical, 24%; biopsies, 57%)
and 58 (cervical, 12%; biopsies, 12%). Seventy-five percent of the HPV16-positive samples were classified into lineages, with
88% defined as lineage A, 10% as lineage D, and 2% as lineage B.
MAIN CONCLUSIONS This study identified a high frequency of European and North American HPV16 lineages, consistent with
the genetic background of the human population in southern Brazil.
Keywords
HPV; lineage; HPV16; intratype variants; Brazil
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