The genus
Mycobacterium
is highly diverse and ubiquitous in nature, comprehending fast- and slow-growing species
with distinct impact in public health. The plasmid-mediated horizontal gene transfer represents one of the major events
in bacteria evolution. Here, we report the complete sequence of a 160,489 bp circular plasmid (pCBMA213_2) from an
atypical and fast-growing environmental mycobacteria. This is a unique plasmid, in comparison with the characterised
mycobacteria plasmids, harboring a type IV-like and ESX-P2 type VII secretion systems. pCBMA213_2 can be further
explored for evolutionary and conjugation studies as well as a tool to manipulate DNA within this bacteria genus.
