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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 112, No. 10, 2017, pp. 655-663
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Bioline Code: oc17094
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 112, No. 10, 2017, pp. 655-663
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In silico analysis of amino acid variation in human respiratory syncytial virus: insights into immunodiagnostics
Souza, Claudemir; Zanchin, Nilson IT; Krieger, Marco A & Ludwig, Adriana
Abstract
BACKGROUND The highly contagious nature of human respiratory syncytial virus (HRSV) and the gravity of its infection
in newborns and vulnerable adults pose a serious public health problem. Thus, a rapid and sensitive diagnostic test for viral
detection that can be implemented upon the first appearance of symptoms is needed. The genetic variation of the virus must be
considered for immunodiagnostic purposes.
OBJECTIVES To analyse HRSV genetic variation and discuss the possible consequences for capture immunoassay development.
METHODS We performed a wide analysis of N, F and G protein variation based on the HRSV sequences currently available in
the GenBank database. We also evaluated their similarity with homologous proteins from other viruses.
FINDINGS The mean amino acid divergences for the N, F, and G proteins between HRSV-A and HRSV-B were determined to be
approximately 4%, 10% and 47%, respectively. Due to their high conservation, assays based on the full-length N and F proteins
may not distinguish HRSV from human metapneumovirus and other Mononegavirales viruses, and the full-length G protein
would most likely produce false negative results due to its high divergence.
MAIN CONCLUSIONS We have identified specific regions in each of these three proteins that have higher potential to produce
specific results, and their combined utilisation should be considered for immunoassay development.
Keywords
HRSV; genetic variability; diagnostics; antigen detection
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