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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 112, No. 10, 2017, pp. 664-673
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Bioline Code: oc17095
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 112, No. 10, 2017, pp. 664-673
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Morphologic study of the effect of iron on pseudocyst formation in Trichomonas vaginalis and its interaction with human epithelial cells
Dias-Lopes, Geovane; Saboia-Vahia, Leonardo; Margotti, Eliane Trindade; Fernandes, Nilma de Souza; Castro, Cássia Luana de Faria; Oliveira-Junior, Francisco Odencio; Peixoto, Juliana Figueiredo; Britto, Constança; Silva-Filho, Fernando Costa e; Cuervo, Patricia & Jesus, José Batista de
Abstract
BACKGROUND Trichomonas vaginalis is the aetiological agent of human trichomoniasis, which is one of the most prevalent
sexually transmitted diseases in humans. Iron is an important element for the survival of this parasite and the colonisation of the
host urogenital tract.
OBJECTIVES In this study, we investigated the effects of iron on parasite proliferation in the dynamics of pseudocyst formation
and morphologically characterised iron depletion-induced pseudocysts.
METHODS We performed structural and ultrastructural analyses using light microscopy, scanning electron microscopy and
transmission electron microscopy.
FINDINGS It was observed that iron depletion (i) interrupts the proliferation of T. vaginalis, (ii) induces morphological changes
in typical multiplicative trophozoites to spherical non-proliferative, non-motile pseudocysts, and (iii) induces the arrest of cell
division at different stages of the cell cycle; (iv) iron is the fundamental element for the maintenance of typical trophozoite
morphology; (v) pseudocysts induced by iron depletion are viable and reversible forms; and, finally, (vi) we demonstrated that
pseudocysts induced by iron depletion are able to interact with human epithelial cells maintaining their spherical forms.
MAIN CONCLUSIONS Together, these data suggest that pseudocysts could be induced as a response to iron nutritional stress and
could have a potential role in the transmission and infection of T. vaginalis.
Keywords
Trichomonas vaginalis; iron; pseudocyst; morphological transformation; trophozoites; human epithelial cells
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