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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 112, No. 11, 2017, pp. 785-7889
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Bioline Code: oc17112
Full paper language: English
Document type: Short Communication
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 112, No. 11, 2017, pp. 785-7889
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Construction of Mycobacterium tuberculosis cdd knockout and evaluation of invasion and growth in macrophages
Villela, Anne Drumond; Rodrigues-Junior, Valnês S; Pinto, Antônio Frederico Michel; Falcão, Virgínia Carla de Almeida; Sánchez-Quitian, Zilpa Adriana; Eichler, Paula; Bizarro, Cristiano Valim; Basso, Luiz Augusto & Santos, Diógenes Santiago
Abstract
Cytidine deaminase (MtCDA), encoded by cdd gene (Rv3315c), is the only enzyme identified in nucleotide biosynthesis
pathway of Mycobacterium tuberculosis that is able to recycle cytidine and deoxycytidine. An M. tuberculosis
knockout strain for cdd gene was obtained by allelic replacement. Evaluation of mRNA expression validated cdd deletion
and showed the absence of polar effect. MudPIT LC-MS/MS data indicated thymidine phosphorylase expression was
decreased in knockout and complemented strains. The cdd disruption does not affect M. tuberculosis growth both in Middlebrook
7H9 and in RAW 264.7 cells, which indicates that cdd is not important for macrophage invasion and virulence.
Keywords
Mycobacterium tuberculosis; cdd gene; cytidine deaminase
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