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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 112, No. 12, 2017, pp. 829-837
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Bioline Code: oc17120
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 112, No. 12, 2017, pp. 829-837
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Dengue serotype-specific immune response in Aedes aegypti and Aedes albopictus
Smartt, Chelsea T; Shin, Dongyoung & Alto, Barry W
Abstract
BACKGROUND Dengue viruses (DENV) are considered one of the most important emerging pathogens and dengue disease is a
global health threat. The geographic expansion of dengue viruses has led to co-circulation of all four dengue serotypes making
it imperative that new DENV control strategies be devised.
OBJECTIVES Here we characterize dengue serotype-specific innate immune responses in Aedes aegypti and Aedes albopictus
using DENV from Puerto Rico (PR).
METHODS Ae. aegypti and Ae. albopictus were infected with dengue serotype 1 and 2 isolated from Puerto Rico. DENV infected
mosquito samples were collected and temporal change in expression of selected innate immune response pathway genes analyzed
by quantitative real time PCR.
FINDINGS The Toll pathway is involved in anti-dengue response in Ae. aegypti, and Ae. albopictus. Infections with PR DENV-1 elicited a stronger response from genes of the Toll immune pathway than PR DENV-2 in Ae. aegypti but in infected Ae.
albopictus expression of Toll pathway genes tended to be similar between the serotypes. Two genes (a ribosomal S5 protein gene
and a nimrod-like gene) from Ae. albopictus were expressed in response to DENV.
MAIN CONCLUSIONS These studies revealed a role for antiviral genes in DENV serotype-specific interactions with DENV
vectors, demonstrated that infections with DENV-2 can modulate the Toll immune response pathway in Ae. aegypti and elucidated
candidate molecules that might be used to interfere with serotype specific vector-virus interactions.
Keywords
dengue; serotype; Aedes aegypti; Aedes albopictus; antiviral response
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