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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060 EISSN: 1678-8060
Vol. 113, No. 1, 2018, pp. 1-4
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Bioline Code: oc18004
Full paper language: English
Document type: Research Article
Document available free of charge
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Memórias do Instituto Oswaldo Cruz, Vol. 113, No. 1, 2018, pp. 1-4
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Detection of occult hepatitis B in serum and oral fluid samples
Portilho, Moyra Machado; Nabuco, Leticia Cancella; Nogueira, Cristiane Alves; Brandão-Mello, Carlos Eduardo; Pilotto, José Henrique; Flores, Geane Lopes; Lewis-Ximenez, Lia Laura; Lampe, Elisabeth & Villar, Livia Melo
Abstract
In occult hepatitis B infection (OBI), hepatitis B virus DNA (HBV DNA) can be detected in serum samples; however, oral fluid
collection for detection of HBV DNA has not yet been explored, despite the availability of collection devices. Serum and oral
fluid samples from 45 hepatitis B core antibody (anti-HBc)-positive patients were collected for the amplification of the HBV
polymerase gene. HBV DNA was detected in five serum and four oral fluid samples (the detection limit for oral fluid was 1.656
log IU/mL in paired serum). In conclusion, simple methodologies of sample collection and in-house polymerase chain reaction
(PCR) allowed detection of HBV DNA, and these could be used to improve the diagnosis of OBI, especially in locations with
limited resources.
Keywords
occult hepatitis B; HBV DNA; oral fluid
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