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Characterisation of a rare, reassortant human G10P[14] rotavirus strain detected in Honduras
Quaye, Osbourne; Roy, Sunando; Rungsrisuriyachai, Kunchala; Esona, Mathew D; Xu, Ziqian; Tam, Ka Ian; Banegas, Dina J Castro; Rey-Benito, Gloria & Bowen, Michael D
Abstract
BACKGROUND Although first detected in animals, the rare rotavirus strain G10P[14] has been sporadically detected in humans
in Slovenia, Thailand, United Kingdom and Australia among other countries. Earlier studies suggest that the strains found in
humans resulted from interspecies transmission and reassortment between human and bovine rotavirus strains.
OBJECTIVES In this study, a G10P[14] rotavirus genotype detected in a human stool sample in Honduras during the 2010-2011
rotavirus season, from an unvaccinated 30-month old boy who reported at the hospital with severe diarrhea and vomiting, was
characterised to determine the possible evolutionary origin of the rare strain.
METHODS For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence
independent amplification. The amplicons were sequenced by next-generation sequencing using the Illumina MiSeq 150 paired
end method. The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed
using PhyML version 3.0.
FINDINGS The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed
classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. Six of the genes (VP1, VP2, VP3, VP6, NSP2 and NSP4) were
DS-1-like. NSP1 and NSP5 were AU-1-like and NSP3 was T6, which suggests that multiple reassortment events occurred in the
evolution of the strain. The phylogenetic analyses and genetic distance calculations showed that the VP7, VP4, VP6, VP1, VP3,
NSP1, NSP3 and NSP4 genes clustered predominantly with bovine strains. NSP2 and VP2 genes were most closely related to
simian and human strains, respectively, and NSP5 was most closely related to a rhesus strain.
MAIN CONCLUSIONS The genetic characterisation of the G10P[14] strain from Honduras suggests that its genome resulted from
multiple reassortment events which were possibly mediated through interspecies transmissions.
Keywords
rotavirus; viral genome; viral proteins; non-structural proteins; reassortant
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