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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 89, No. 1, 1994, pp. 53-58
Bioline Code: oc94010
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 89, No. 1, 1994, pp. 53-58

 en Standardization and Evaluation of Elisa for the Serodiagnosis of Amoebic Liver Abscess
R Santiago Nicholls; Marcos I Restrepo; Sofia Duque; M Consuelo Lopez & Augusto Corredor

Abstract

An ELISA test for the serological diagnosis of amoebic liver
abscess (ALA) was standardized and evaluated in sera from
three groups of patients: (1) three patients with diagnosis
confirmed by isolation of the parasite, (2) thirty seven
patients with diagnosis established by clinical findings and
ultrasound studies and (3) seven patients whose diagnosis were
established by clinical findings and a positive double
immunodifusion test. Ninety one serum samples from healthy
subjects and 22 from patients with other liver or parasitic
diseases were also included in the study. 

The optimum concentration of Entamoeba histolytica antigen was 1.25 ug/ml and optimum dilutions of serum and anti-human IgG -alkaline phosphatase conjugate were 1:400 and 1:4000 respectively. The cut-off point of the ELISA test in this study was an absorbance value of 0.34. The test parameters were: sensitivity = 95.7%, specificity = 100%, positive predictive value = 100% and negative predictive value = 98.2%.

The ELISA test was found to be of great use as a diagnostic tool for the establishment of amoebic etiology in patients with clinical supposition of ALA. The test could also be used for seroepidemiological surveys of the prevalence of invasive amoebiasis in a given population, since it allows the processing of a greater number of samples at a lower cost than other serological tests.

Keywords
amoebic liver abscess - ELISA - serodiagnosis amoebiasis

 
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