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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 89, No. 2, 1994, pp. 183-187
Bioline Code: oc94038
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 89, No. 2, 1994, pp. 183-187

 en Comparative Evaluation of a Simple and Sensitive Assay for Detection of Orthomyxo and Paramyxoviruses
JNSS Couceiro; ESS Couceiro; AMV Pinto; RD Machado & MC Cabral

Abstract

Studies were done to evaluate comparatively the traditional HA
assay and a more recently introduced lectin-neuraminidase (LN)
methodology in search of a simple and sensitive assay for virus
detection during laboratorial diagnosis. The results proved the
value of LN assay as a sensitive methodology for detection of virus
particles, presenting results at least equal to those obtained by HA
(hemagglutination) assay, with significant values of accumulated
frequencies for LN/HA factors (ratios between LN and HA titers)
higher than two. The accumulated values of frequencies for LN/HA
factors as high as four were very significant, 72.7% for
influenzavirus and 60.7% for Newcastle disease virus (NDV), moreover
accumulated frequencies for LN/HA factors even as high as 32 were
due to influenzavirus (45.4%) and NDV (7.2%) samples. After the
storage period, most of those concentraded samples that even did not
present HA titers could be detected through LN assay, demonstrating
a lower threshold for virus detection.

Keywords
hemagglutination assay - lectin-neuraminidase assay - influenzaviruses - parainfluenzaviruses

 
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