Ferromagnetic dacron is proposed as an alternative solid-phase
for magnetic enzyme immunoassays. Human serum albumin (HSA) was
covalently immobilized onto ferromagnetic dacron and an enzyme
immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-
phenylenediamine (OPD) and hydrogen peroxide were used as the
enzymatic label and substrates, respectively. Best results were
observed when particles of 63-100 um (diameter) and 10 ug of
immobilized antigen were used. Positive reactions were detected
until dilutions of 1:51200 of immune sera. Its reproducibility was
similar to standard ELISA. Disruption of the immunocomplexes formed
and recuperation of the immobilized antigen in other immunoassays
also proved to be reliable.
