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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 89, No. 2, 1994, pp. 189-193
Bioline Code: oc94039
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 89, No. 2, 1994, pp. 189-193

 en The Use of Ferromagnetic Dacron as Solid-phase in Enzyme Immunoassays
Ana Maria Dos A Carneiro Leao; Luiz B Carvalho Jr & Elizabeth Malagueno

Abstract

Ferromagnetic dacron is proposed as an alternative solid-phase
for magnetic enzyme immunoassays. Human serum albumin (HSA) was
covalently immobilized onto ferromagnetic dacron and an enzyme
immunoassay was developed using anti-HSA rabbit sera. Peroxidase, o-
phenylenediamine (OPD) and hydrogen peroxide were used as the
enzymatic label and substrates, respectively. Best results were
observed when particles of 63-100 um (diameter) and 10 ug of
immobilized antigen were used. Positive reactions were detected
until dilutions of 1:51200 of immune sera. Its reproducibility was
similar to standard ELISA. Disruption of the immunocomplexes formed
and recuperation of the immobilized antigen in other immunoassays
also proved to be reliable.

Keywords
magnetic ELISA - ferromagnetic dacron - human serum albumin

 
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