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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 89, No. 2, 1994, pp. 195-202
Bioline Code: oc94040
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 89, No. 2, 1994, pp. 195-202

 en Human Papillomavirus Detection in Genital Lesions by in Situ Hybridization and Ultrastructural Observations
LHS Oliveira; ICPP Frugulhetti; MRL Passos; SMB Cavalcanti & MEF Fonseca


Detection of papillomavirus DNA in situ hybridization technique
was performed in 29 symptomatic patients (6 males and 23 females)
during the period of 1989-1991 at the Clinic for Sexually
Transmitted Diseases, Universidade Federal Fluminense, State of Rio
de Janeiro. All the male patients had condyloma acuminata. Only HPV
6/11 were found in these lesions. Clinical features in the female
patients included vulvar condyloma acuminata, bowenoid papulosis,
flat cervical condyloma, cervical condyloma acuminatum and cervical
intraepithelial neoplasia grade II (CIN II). We also found cases of
condyloma acuminata associated to vulvar intraepithelial neoplasia
grade III (VIN III), as well as to vaginal invasive carcinoma. HPV
6/11 and 16/18 were found in vulvar condyloma acuminata. Mixed
infection by 6/11-16/18 HPV were also seen in these lesions as well
as in the patient who had cervical condyloma acuminatum. HPV 16/18
were found in the condyloma acuminatum plus VIN III and in the CIN
II lesions. We have found HPV 31/33/51 in the specimen of condyloma
acuminatum plus invasive carcinoma.

In order to investigate the ultrastructural aspects of HPV infection in genital tissue, the biopsies of three female patients were observed under electron microscope. Mature virus particles were found in the cells of a condyloma acuminatum as well as in the condyloma acuminatum plus invasive carcinoma case. In another sample, chromosome breakages were found in the nuclei of the infected cells although no viral particles were observed.

papillomavirus - viral ultrastructure - in situ hybridization

© Copyright 1994 Memorias do Instituto Oswaldo Cruz.
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