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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 2, 1995, pp. 211-213
Bioline Code: oc95042
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 2, 1995, pp. 211-213

 en DNA Polymorphism of Schistosomes and their Snail Hosts
Andrew JG Simpson; Emmanuel Dias Neto; Teofania Hda Vidigal; Heloisa B Pena; Omar S Carvalho & Sergio DJ Pena

Abstract

Analysis of the genomes of schistosomes and one of their
intermediate hosts, Biomphalaria glabrata, using Random
Amplified Polymorphic DNA (RAPD) demonstrated that
intraspecific genetic polymorphism  in the parasite is limited
but in the snail is highly pronounced. This  suggests an
important role for the snail in the determination of the
epidemiology of the disease. In addition to their
intraspecific stability, schistosome derived RAPDs exhibit a
high level of interspecific polymorphism and are thus ideal
for the construction of phylogenetic trees. For the detection
of intraspecific polymorphisms extensive variation in the
mitochondrial DNA is being exploited  for the development of a
PCR based test for Schistosoma mansoni. Gene level
polymorphims are being analyzed by Low Stringency Single
Specific Primer PCR.

Keywords
schistosomes - Biomphalaria glabrata - DNA - polymorphisms - PCR

 
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