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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 2, 1995, pp. 215-216
Bioline Code: oc95043
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 2, 1995, pp. 215-216

 en Sequencing and Identification of Expressed Schistosoma mansoni check for this species in other resources Genes by Random Selection of cDNA Clones from a Directional Library
Gloria R Franco; Andrew JG Simpson & Sergio DJ Pena

Abstract

We have initiated a gene discovery program in  Schistosoma  mansoni
  check for this species in other resources
 based on
the technique of Expressed Sequence Tags (ESTs), i.e. partial sequences of 
cDNAs obtained from single passes in automatic DNA sequencers.
ESTs can be used to identify genes on the basis of their homology with 
sequences from other species deposited in DNA or protein databases. 
Transcripts with sequences without matches in the databases may represent 
novel parasite-specific genes. This approach has shown to be very efficient 
and in less than two years a broad range of novel genes has already been 
ascertained, more than doubling the number of known S. mansoni genes.

Keywords
Schistosoma mansoni - automated DNA sequencing - EST - gene identification

 
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