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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 2, 1995, pp. 249-253
Bioline Code: oc95049
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 2, 1995, pp. 249-253

 en Comparison of purified 12 kDa and recombinant 15 kDa Fasciola hepatica Antigens Related to a Schistosoma mansoni Fatty Acid Binding Protein
George V Hillyer

Abstract

Vaccines in schistosomiasis using homologous antigens have
been studied extensively in experimentally infected mammalian hosts.
Vaccines using heterologous antigens have received comparatively less
attention.  This review summarizes recent work on a heterologous 12 kDa 
Fasciola hepatica antigenic polypeptide which cross reacts with 
Schistosoma mansoni. A cDNA has been cloned and sequenced, and the 
predicted amino acid sequence of the recombinant protein has been shown 
to have significant (44 %) identity with a 14 kDa S. mansoni fatty 
acid binding protein. Thus in the parasitic trematodes fatty acid binding 
proteins may be potential vaccine candidates. The F. hepatica recombinant 
protein has been overexpressed and purified and denoted rFh15. Preliminary 
studies show that rFh15 migrates more slowly (i.e. may be slightly larger) 
than nFh12 on SDS-PAGE and has a predicted pI of 6.01 vs. observed pI of 
5.45.  Mice infected with F. hepatica develop antibodies to nFh12 by 2 
weeks of infection vs. 6 weeks of infection to rFh15; on the other hand, 
mice with schistosomiasis mansoni develop antibodies to both nFh12 and 
rFh15 by 6 weeks of infection. Both the F. hepatica and S. mansoni
cross-reactive antigens may be cross-protective antigens
with the protection inducing capability against both species.

Keywords
Fasciola hepatica - Schistosoma mansoni - heterologous resistance - Fasciola/Schistosoma cross-reactivity - fatty acid binding protein

 
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