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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 3, 1995, pp. 353-357
Bioline Code: oc95071
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 3, 1995, pp. 353-357

 en Genetic variability and differentiation between populations of Rhodnius prolixus and R. pallescens, vectors of chagas' disease in colombia
German Lopez & Jaime Moreno


Enzyme polymorphism in Rhodnius prolixus and R. pallescens
(Hemiptera, Reduviidae), principal vectors of Chagas' disease in
Colombia, was analyzed using starch gel electrophoresis. Three
geographic locations were sampled in order to determine gene flow
between populations and to characterize intra- and interspecific
differences. Of 25 enzymes assayed 10 were successfully resolved
and then used to score the genetic variation. The enzymes PEPD,
GPI, PGM and ICD were useful to differentiate these species and
PGD, PGM and MDH distinguished between sylvatic and domiciliary
populations of R. prolixus. Both polymorphism and heterozygosity
indicated greater genetic variability in sylvatic habitats (H=
0.021) compared to domiciliary habitats (H=0.006) in both
species. Geneflow between sylvatic and domiciliary populations
in R. prolixus was found to be minimal. This fact and the genetic
distance between them suggest a process of genetic isolation in
the domiciliary population.

Rhodnius prolixus - Rhodnius pallescens - isoenzyme polymorphism - Chagas' disease - Triatominae - Colombia

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