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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 3, 1995, pp. 407-410
Bioline Code: oc95079
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 3, 1995, pp. 407-410

 en Partial inhibition of hemocyte agglutination by Lathyrus odoratus lectin in Crassostrea virginica infected with Perkinsus marinus
Thomas C Cheng & William J Dougherty

Abstract

Quantitative determinations of agglutination of
hemocytes from oysters, Crassostrea virginica, by the
Lathyms odoratus lectin at five concentrations revealed
that clumping of hemocytes from oysters infected with
Perkinsus mannus is partially inhibited. Although the
nature of the hemocyte surface saccharide, which is not
D(+)-glucose, D(+)mannose, or alpha-methyl-D-mannoside,
remains to be determined it may be concluded that this
molecule also occurs on the surface of P. marinus.

It has been demonstrated that the panning technique (Ford et al. 1990) is qualitatively as effective for determining the presence of P. marinus in C. virginica as the hemolymph assay method (Gauthier & Fisher 1990).

Keywords
Crassostrea virginica - oyster - Perkinsus marinus - Lathyrus odoratus - lectin - hemocytes

 
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