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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 90, No. 4, 1995, pp. 547-551
Bioline Code: oc95109
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 90, No. 4, 1995, pp. 547-551

 en Initiation of primary cell cultures from embryos of the mosquitoes Anopheles albimanus and Aedes taeniorhynchus (diptera: culicidae)
Felio J Bello Garcia; Jorge Boshell; Gloria Rey; Alberto Morales & Victor Alberto Olano

Abstract

Primary cell cultures were obtained from eggs of Anopheles
albimanus and Aedes taeniorhynchus mosquitoes, vectors of
human malaria and of Venezuelan equine encephalitis virus,
respectively. The cellular growth of the An. albimanus cells
began four weeks after explanting the embryonic tissues in
MK/VP12 medium, supplemented with 15% fetal bovine serum. The
culture showed heterogeneous cellular morphology. With regard
to the Ae. taeniorhynchus culture, cellular growth occurred
three weeks after initiating the culture in MM/VP12 medium.
The majority of cells were small and round. Karyotypes were
examined in the latter species.

Keywords
mosquitoes - cell cultures - culture media - karyotype

 
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