Primary cell cultures were obtained from eggs of Anopheles
albimanus and Aedes taeniorhynchus mosquitoes, vectors of
human malaria and of Venezuelan equine encephalitis virus,
respectively. The cellular growth of the An. albimanus cells
began four weeks after explanting the embryonic tissues in
MK/VP12 medium, supplemented with 15% fetal bovine serum. The
culture showed heterogeneous cellular morphology. With regard
to the Ae. taeniorhynchus culture, cellular growth occurred
three weeks after initiating the culture in MM/VP12 medium.
The majority of cells were small and round. Karyotypes were
examined in the latter species.