search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations




Vol. 91, No. 1, 1996, pp. 83-86
Bioline Code: oc96013x
Full paper language: English
Document type: Research Article
Document available free of charge

, Vol. 91, No. 1, 1996, pp. 83-86

 en Diagnosis of Malaria by Acridine Orange Fluorescent Microscopy in an Endemic Area of Venezuela
Boschj, Irene; Bracho, Carmen & Pérez, Hilda A

Abstract

Fluorescent (acridine orange) microscopical examination of capillary centrifuged blood (quantitative buffy coat [QBC®] analysis) and Giemsa stained thick blood smears (GTS) were compared for diagnosis of malaria in blood specimens from adults living in malaria transmission areas of the States of Bolivar and Amazonas in southeastern and south Venezuela, respectively. Of a total of 198 GTS examined, 95 subjects (48%) showed parasitaemia. Among the 95 blood films with a positive GTS, 94 were judged positive by the QBC. However, positive QBC tubes were found in 29 out of 103 blood specimens with a negative GTS. Thus, relative to a GTS standard, the sensitivity and specificity of the QBC-test was 99.2% and 72%, respectively. Young trophozoites of  Plasmodium  vivax
  check for this species in other resources
 and P. falciparum could not be distinguished with certainty. It is confirmed that the QBC offers many advantages compared with the standard diagnosis of malaria parasites, specifically in the speed of staining and ease of interpretation. However, in places where P. falciparum and P. vivax occur, species and stage differentiation should be confirmed with the GTS.

Keywords
diagnosis - QBC test - Plasmodium vivax - Plasmodium falciparum

 
© Copyright 1996 Fundacao Oswaldo Cruz

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2017, Site last up-dated on 16-Oct-2017.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil