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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 91, No. 1, 1996
Bioline Code: oc96026
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 91, No. 1, 1996

 en RESEARCH NOTE - A note on slide-mounting technique of unfed immature stages of amblyomma cajennense (fabricius, 1787) (acari: ixodidae)
Famadas, Katia Maria; da Serra-Freire, Nicolau Maues & Faccini, Joao Luiz Horacio

Abstract

Several tick slide-mounting techniques have been used for many years with varying degrees of success. The classic Canada balsam technique employed by entomologists and acarologists over many decades is efficient but time consuming (RL Palma 1978 New Zeal Entomol 6: 169-170). The classic methods used in Acarology include the water soluble media such as Berlese, Faure and Hoyer (GM Krantz 1978 A Manual of Acarology, Oregon State University Book Store, 2nd ed., Oregon, 509pp., A Fain 1980 Inter J Acarol 6: 169-170). Water soluble media are inadequate in the humid tropic, even when the slides are ringed with a water-proofing material such as Glyptal. Crystallization of the media, probably due to absorption of water, is the main problem with slide-mounting specimens.

Keywords
slide-mounting technique - Canada balsam medium - Amblyomma cajennense - Ixodidae

 
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