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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 91, No. 2, 1996, pp. 147-151
Bioline Code: oc96028
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 91, No. 2, 1996, pp. 147-151

 en Human Parvovirus B19 Infection and Hydrops Fetalis in Rio de Janeiro, Brazil
Rita CN Cubel; Aparecida GP Garcia; Claudia S Pegado; Hilda I Ramos; Maria EF Fonseca; Jonhatan P Clewley; Bernard J Cohen & Jussara P Nascimento

Abstract

Formalin-fixed paraffin embedded lung and liver tissue from
23 cases of non immune hydrops fetalis and five control cases,
in which hydrops were due to syphilis (3) and  genetic causes
(2), were examined for the presence of human parvovirus B19 by
DNA hybridisation. Using in situ hybridisation with a
biotynilated probe one positive case was detected.  Using 32P-
labelled probes in a dot blot assay format, five further
positives were obtained.  These were all confirmed as positive
by a nested polymerase chain reaction assay.  Electron
microscopy revealed virus in all these five positive cases. 
The six B19 DNA positive cases of hydrops fetalis were from
1974, 1980, 1982, 1987 and 1988, four of which occurred during
the second half of the year, confirming the seasonality of the
disease.

Keywords
human parvovirus B19 hydrops fetalis visrus detection

 
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