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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 91, No. 2, 1996, pp. 195-198
Bioline Code: oc96039
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 91, No. 2, 1996, pp. 195-198

 en The Use of Polyvinyl Alcohol Glutaraldehyde as Solid-phase in ELISA for Plague
Aureci Maria Araujo; Andre Tavares S Petribu; Gustavo Henrique T Sales Barbosa; Jose Ricardo P Diniz; Alzira MP de Almeida; Walter Mendes Azevedo; Elizabeth Malagueno & Luiz B Carvalho Jr

Abstract

Discs of polyvinyl alcohol cross-linked with glutaraldehyde
were synthesized under acid catalysis (H2SO4). Then, the
antigen F1 purified from Yersinia pestis was covalently linked
to this modified polymer. Afterwards, an enzyme-linked
immunosorbent assay (ELISA) was established for the diagnosis
of plague in rabbit and human. The best conditions for the
method were achieved by using 1.3 ug of F1 prepared in 0.067 M
phosphate buffer, pH 7.2, containing 1 M NaCl (PBS); anti-IgG
peroxidase conjugate diluted 6,000 times and as a blocking
agent 3% w/v skim milk in PBS. The titration of positive
rabbit serum according to this procedure detected antibody
concentrations up to 1:12,800 times. The present method, the
conventional ELISA and passive haemagglutination assay are
compared.

Keywords
polyvinyl alcohol glutaraldehyde Yersinia pestis ELISA Brazil

 
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