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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 91, No. 3, 1996, pp. 279-284
Bioline Code: oc96056
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 91, No. 3, 1996, pp. 279-284

 en An Oligonucleotide Probe Derived from kDNA Minirepeats is Specific for Leishmania (Viannia)
Octavio Fernandes; Marcelo Bozza; Juan M Pascale; Antonio B de Miranda; Ulisses G Lopes & Wim M Degrave

Abstract

Sequence analysis of Leishmania (Viannia) kDNA
minicircles and analysis of multiple sequence alignments  of  the
conserved region (minirepeats) of five distinct minicircles from
L. (V.) braziliensis species with corresponding sequences
derived from other dermotropic leishmanias  indicated  the
presence of  a  sub-genus specific sequence.  An oligonucleotide
bearing this sequence was designed and used as a molecular probe,
being able to recognize solely the sub-genus Viannia
species in hybridization experiments.  A dendrogram reflecting
the homologies among the minirepeat sequences was constructed.
Sequence clustering was obtained corresponding to the traditional
classification based on similarity of biochemical, biological and
parasitological characteristics of these Leishmania
species, distinguishing the Old World dermotropic leishmanias,
the New World dermotropic leishmanias of the sub-genus
Leishmania and of the sub-genus Viannia.

Keywords
Leishmania (Viannia) identification - minicircle DNA sequences - oligonucleotide probe - Leishmania braziliensis - Leishmania panamensis

 
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