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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 91, No. 4, 1996, pp. 501-505
Bioline Code: oc96096
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 91, No. 4, 1996, pp. 501-505

 en The Haemoculture of Trypanosoma minasense Chagas, 1908
Mariangela Ziccardi; Ricardo Lourenco-de-Oliveira & Ricardo Nogueira

Abstract

Trypanosoma minasense was isolated for the first time
in blood axenic culture from a naturally infected marmoset,
Callithrix penicillata, from Brazil. The parasite grew
profusely in an overlay of Roswell Park Memorial Institute
medium plus 20% foetal bovine serum, on Novy, McNeal and
Nicolle medium (NNN), at 27 C, with a peak around 168 hr.

The morphometry of cultural forms of T. minasense, estimates of cell population size and comparative growth in four different media overlays always with NNN, were studied. The infectivity of cultural forms to marmosets (C. penicillata and C. jacchus) and transformation of epimastigotes into metacyclic-like forms in axenic culture in the presence of chitin derivates (chitosan) were evaluated.

Keywords
Trypanosoma minasense - neotropical primates - trypanosomes - marmosets - trypanosomatidae flagellates - culture - Brazil

 
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