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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 91, No. 4, 1996, pp. 507-514
Bioline Code: oc96097
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 91, No. 4, 1996, pp. 507-514

 en Biological Characterization of Clones Derived from the Edmonston Strain of Measles Virus in Comparison with Schwarz and CAM-70 Vaccine Strains
Maria Beatriz Junqueira Borges; George F Mann & Marcos da Silva Freire

Abstract

Four virus clones were derived from the Edmonston strain of
measles virus by repeated plaque purification.  These clones
were compared with the vaccine strains Schwarz and CAM-70 in
terms of biological activities including plaque formation,
hemagglutination, hemolysis and replication in Vero cells and
chick embryo fibroblasts (CEF).

Two clones of intermediate plaque yielded mixed plaque populations on subcultivation whereas the other two, showing small and large plaque sizes, showed stable plaque phenotypes. The vaccine strains showed consistent homogeneous plaque populations.

All the Edmonston clones showed agglutination of monkey erythrocytes in isotonic solution while both vaccine strains hemagglutinated only in the presence of high salt concentrations.

Variation in the hemolytic activity was observed among the four clones but no hemolytic activity was detected for the vaccine virus strains.

Vaccine strains replicated efficiently both in Vero cells and CEF. All four clones showed efficient replication in Vero cells but different replication profiles in CEF. Two of them replicated efficiently, one was of intermediate efficiency and the other showed no replication in CEF.

Two of the clones showed characteristics similar to vaccine strains. One in terms of size and homogeneity of plaques, the other for a low hemolytic activity and both for the efficiency of propagation in CEF.

Keywords
biological characterization - plaque variants - measles - vaccine

 
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