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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 1, 1997, pp. 21-26
Bioline Code: oc97004
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 1, 1997, pp. 21-26

 en Seroepidemiological studies of cutaneous Leishmaniasis in the Campeche State of Mexico
Amalia Monroy-Ostria; Tayde Sosa-Cabrera; Beatriz Rivas-Sanchez; Rosaura Ruiz-Tuyu; Ana R Mendoza-Gonzalez & Luis Favila-Castillo

Abstract

Seroepidemiological studies of cutaneous leishmaniasis were carried
out on 169 individuals in a rural area of the Campeche state of
Mexico. Fifty showed cutaneous lesions suggestive of leishmaniasis,
70% were parasite positive and 96% skin test positive. An overall 40%
positivity to skin test with Montenegro's antigen was found. Most of the 
affected individuals were males from 11 to 30 years-old. Antibodies were
determined by immunofluorescent antibody test (IFA) and by Western blot.
Two antigen preparations were used, one from a Leishmania mexicana
strain which produced localized cutaneous leishmaniasis (LCL) and the
other from a diffuse cutaneous leishmaniasis (DCL). In the general
population from the area of study 19% gave positive IFA tests with DCL
antigen and 20% with LCL antigen while for the patients 67% gave positive
IFA tests with DCL and 71% with LCL. By Western blot analysis most of the
patients recognized more antigens in the DCL than in the LCL strain. In
the DCL strain 78% of patients recognized a 105 kDa, 34% a 139 kDa, 28% a
117 kDa and 26% a 205 kDa MW antigen. In the LCL strain 40% of patients
recognized a 205 kDa and 22% a 175 kDa antigens.

Keywords
seroepidemiology - cutaneous leishmaniasis - Campeche state - Mexico - Western blot

 
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