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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 1, 1997, pp. 97-100
Bioline Code: oc97020
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 1, 1997, pp. 97-100

 en Detection of rotavirus in sewage and creek water: efficiency of the concentration method
DU Mehnert; KE Stewien; CM Harsi; APS Queiroz; JMG Candeias & JAN Candeias


Simian rotavirus SA-11, experimentally seeded, was recovered from raw
domestic sewage by a two-step concentration procedure, using filtration
through a positively charged microporous filter (Zeta Plus 60 S) followed
by ultracentrifugation, effecting an 8,000-fold concentration. By this
method, a mean recovery of 81% 7.5 of the SA-11 virus, was achieved.

rotavirus - sewage - virus concentration - direct immunoperoxidase

© Copyright 1997 Fundacao Oswaldo Cruz
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