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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 1, 1997, pp. 101-106
Bioline Code: oc97021
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 1, 1997, pp. 101-106

 en Specific identification of Biomphalaria tenagophila and Biomphalaria occidentalis populations by the low stringency polymerase chain reaction
Edina Rodrigues Pires; Teofania HDA Vidigal; Horacio MS Teles; Andrew JG Simpson & Omar S Carvalho


Although Biomphalaria occidentalis and B. tenagophila are
indistinguishable on the basis of shell morphology and the majority of
their genital organs, only the latter is susceptible to infection with
Schistosoma mansoni. Thus, the identification of these species is 
fundamental to epidemiological studies of schistosomiasis. Here we describe
a simple and rapid method for differentiating B. tenagophila from
B. occidentalis based on low stringency polymerase chain reaction
and using a pair of primers specific for the amplification of the 18S rRNA
gene. Analysis of the low stringency product profiles of populations of
these snails from different geographical regions confirmed this approach 
as being applicable to the identification of B. tenagophila and
B. occidentalis in cases where classical morphology is inconclusive.

polymerase chain reaction - low stringency - Biomphalaria tenagophila - Biomphalaria occidentalis - schistosomiasis

© Copyright 1997 Fundacao Oswaldo Cruz
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