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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 5, 1997, pp. 595-600
Bioline Code: oc97111
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 5, 1997, pp. 595-600

 en Diagnosis of dengue by using Reverse Transcriptase- Polymerase Chain Reaction
Marize Pereira Miagostovich; Flavia Barreto dos Santos; Eliane Saraiva M de Araujo; Juarez Dias; Hermann G Schatzmayr & Rita Maria Ribeiro Nogueira

Abstract

A rapid identification of dengue viruses from clinical samples by using a
nested reverse transcriptase-polymerase chain reaction (RT-PCR) procedure
was carried out for diagnostic and epidemiological purposes. RT-PCR
identified DEN-1 and DEN-2 viruses in 41% (41/100) of previously confirmed
cases and provided an accurate confirmation of DHF in four fatal cases.
RT-PCR was also useful for detecting and typing dengue viruses in suspected
cases, allowing a rapid identification of new serotypes in endemic areas.

Keywords
dengue - diagnosis - virological surveillance - polymerase chain reaction

 
© Copyright 1997 Fundacao Oswaldo Cruz
Alternative site location: http://memorias.ioc.fiocruz.br

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