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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 5, 1997, pp. 643-646
Bioline Code: oc97120
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 5, 1997, pp. 643-646

 en Cloning and characterization of a Schistosoma mansoni cDNA clone with a specific antigenic expression during development in a vertebrate host
Arlete Martins Coelho-Castelo & Vanderlei Rodrigues

Abstract

Approximately 2.0 x 10 cDNA clones of an Schistosoma mansoni lambda gt11
cDNA library were screened in duplicate with serum from infected mice
corresponding to distinct phases of infection. A cDNA clone (7/1) was
isolated and recognized only by seven week serum. The clone was subcloned
in pGEX-2T and Western-blot studies showed a specific antigenic expression
confirming that only serum from the chronic phase is capable of recognizing
this antigen. Dot-hybridization with RNA from different developmental
phases of the parasite showed that the corresponding 7/1 RNA is expressed
in all phases of parasite development in vertebrate hosts.

Keywords
Schistosoms mansoni - antigen specific expression - cDNA - cloning

 
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