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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 6, 1997, pp. 821-828
Bioline Code: oc97154
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 6, 1997, pp. 821-828

 en The Trypanosoma cruzi Genome Project: nuclear karyotype and gene mapping of clone CL Brener
Marcia RM Santos; Maria I Cano; Alejandro Schijman; Hernan Lorenzi; Martin Vazquez; Mariano J Levin; Jose Luis Ramirez; Adeilton Brandao; Wim M Degrave & Jose Franco da Silveira

Abstract

By using improved pulsed field gel electrophoresis conditions, the
molecular karyotype of the reference clone CL Brener selected for
Trypanosoma cruzi genome project was established. A total of 20 uniform
chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp)
were resolved in a single run. The weighted sum of the chromosomal bands
was approximately 87 Mbp. Chromoblots were hybridized with 39 different
homologous probes, 13 of which identified single chromosomes. Several
markers showed linkage and four different linkage groups were identified,
each comprising two markers. Densitometric analysis suggests that most of
the chromosomal bands contain two or more chromosomes representing either
homologous chromosomes and/or heterologous chromosomes with similar sizes.

Keywords
Trypanosoma cruzi - clone CL Brener - genome project - molecular karyotype - chromosome mapping - genetic map

 
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