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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 6, 1997, pp. 843-852
Bioline Code: oc97157
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 6, 1997, pp. 843-852

 en Towards the physical map of the Trypanosoma cruzi nuclear genome: construction of YAC and BAC libraries of the reference clone T. cruzi CL-Brener
I Ferrari; H Lorenzi; MJ Levin; Mr Santos; S Brandariz; JM Requena; A Schijman; M Vazquez; JF da Silveira; C Ben-Dov; C Medrano; S Ghio; P Lopez Bergami; I Cano; B Zingales; TP Urmenyi; E Rondinelli; A Gonzalez; A Cortes; MC Lopez; MC Thomas; C Alonso; JL Ramirez; MA Chiurrillo; R Rangel Aldao; A Brandao; W Degrave; V Perrot; M Saumier; A Billaut; D Cohen & D le Paslier


Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (c) the fact that simple Southern blots of electrophoretic karyotypes can be used to map sequence tagged sites and expressed sequence tags to chromosomal bands. A major drawback to cope with is the complexity of T. cruzi genetics, that hinders the construction of a comprehensive genetic map. As a first step towards physical mapping, we report the construction and partial characterization of a T. cruzi CL-Brener genomic library in yeast artificial chromosomes (YACs) that consists of 2,770 individual YACs with a mean insert size of 365 kb encompassing around 10 genomic equivalents. Two libraries in bacterial artificial chromosomes (BACs) have been constructed, BACI and BACII. Both libraries represent about three genome equivalents. A third BAC library (BAC III) is being constructed. YACs and BACs are invaluable tools for physical mapping. More generally, they have to be considered as a common resource for research in Chagas disease.

Trypanosoma cruzi - genome project - physical map - Yac library - BAC library - sequence tagged sites (STS) - expressed sequence tags (EST)

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