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Memórias do Instituto Oswaldo Cruz
Fundação Oswaldo Cruz, Fiocruz
ISSN: 1678-8060
EISSN: 1678-8060
Vol. 92, No. 6, 1997, pp. 863-866
Bioline Code: oc97160
Full paper language: English
Document type: Research Article
Document available free of charge

Memórias do Instituto Oswaldo Cruz, Vol. 92, No. 6, 1997, pp. 863-866

 en Identification of transcribed sequences (ESTs) in the Trypanosoma cruzi Genome Project
Adeilton Brandao; Turan Urmenyi; Edson Rondinelli; Antonio Gonzalez; Antonio B de Miranda & Wim Degrave


Random single pass sequencing of cDNA fragments, also known as generation
of Expressed Sequence Tags (ESTs), has been highly successful in the study
of the gene content of higher organisms, and forms an integral part of most
genome projects, with the objective to identify new genes and targets for
disease control and prevention and to generate mapping probes. In the
Trypanosoma cruzi genome project, EST sequencing has also been a starting
point, and here we report data on the first 797 sequences obtained, partly
from a CL Brener epimastigote non-normalized library, partly on a
normalized library. Only around 30% of the sequences obtained showed
similarity with Genbank and dbEST databases, half of which with sequences
already reported for T. cruzi.

expressed sequence tags sequencing - normalized cDNA library - Trypanosoma cruzi - clone CL Brener - Parasite Genome Projects

© Copyright 1997 Fundacao Oswaldo Cruz
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