Random single pass sequencing of cDNA fragments, also known as generation
of Expressed Sequence Tags (ESTs), has been highly successful in the study
of the gene content of higher organisms, and forms an integral part of most
genome projects, with the objective to identify new genes and targets for
disease control and prevention and to generate mapping probes. In the
Trypanosoma cruzi genome project, EST sequencing has also been a starting
point, and here we report data on the first 797 sequences obtained, partly
from a CL Brener epimastigote non-normalized library, partly on a
normalized library. Only around 30% of the sequences obtained showed
similarity with Genbank and dbEST databases, half of which with sequences
already reported for T. cruzi.
